Current

• Glowing plants

• Applications of fungal bioluminescence to bioimaging

Accomplished

• Structures of fungal luciferin & luciferase

• Mechanism of fungal bioluminescence

• Full biosynthetic pathway of fungal luciferin

1. Mitiouchkina T et al (2020). Plants with genetically encoded autoluminescence. Nat Biotech, in press.

2. Kotlobay AA, Sarkisyan KS et al (2018). Genetically encodable bioluminescent system from fungi. Proc Natl Acad Sci USA 115 (50), 12728-12732.

3. Kaskova ZM et al (2017). Mechanism and color modulation of fungal bioluminescence. Sci Adv 3 (4), e1602847

4. Purtov KV et al (2015). The Chemical Basis of Fungal Bioluminescence. Angew Chem Int Ed Engl 54 (28), 8124–8128.

Current

• Search for Henlea sp. luciferin

• Search for Fridericia heliota luciferase

Accomplished

• Fridericia heliota luciferin and oxyluciferin

• Mechanism of Fridericia heliota bioluminescence

• New peptide chemistry of Fridericia heliota earthworm

Main publications

1. Petushkov VN et al (2023). Deazaflavin cofactor boosts earthworms Henlea bioluminescence. Org & Biomol Chem 21, 415-427.

2. Dubinnyi MA et al (2015). Novel Mechanism of Bioluminescence: Oxidative Decarboxylation of a Moiety Adjacent to the Light Emitter of Fridericia Luciferin. Angew Chem Int Ed Engl 54 (24), 7065–7067.

3. Dubinnyi MA et al (2014). Novel peptide chemistry in terrestrial animals: Natural luciferin analogues from the bioluminescent earthworm Fridericia heliota. Chem Eur J 21 (10), 3942–3947

4. Petushkov VN et al (2014). A novel type of luciferin from the siberian luminous earthworm Fridericia heliota: Structure elucidation by spectral studies and total synthesis. Angew Chem Int Ed Engl 53 (22), 5566–5568.

Current

• Search for Chaetopterus luciferin

• Biosynthesis of Odontosyllis luciferin

Accomplished

• Recombinant Odontosyllis luciferase

• Structure of Odontosyllis luciferin

Main publications

1. Bolt YV et al (2023). Total Synthesis of Racemic Thieno[3,2-f]thiochromene Tricarboxylate, a Luciferin from Marine Polychaeta Odontosyllis undecimdonta. Org Lett 25(26), 4892–4897.

2. Zagitova RI, Purtov KV et al (2023). Conjugated Dienoic Acid Peroxides as Substrates in Chaetopterus Bioluminescence System. Intl J Mol Sci 24, 9466.

3. Kotlobay AA et al (2019). Bioluminescence chemistry of fireworm Odontosyllis. Proc Natl Acad Sci USA 116(38), 18911-18916.

4. Schultz DT et al (2018). Luciferase of the Japanese syllid polychaete Odontosyllis umdecimdonta. Biochem Biophys Res Comm 502(3), 318-323.

Current

• Search for Keroplatus bioluminescence components

• Search for Keroplatus and Orfelia BL similarities

Accomplished

• Structure of Keroplatus oxyluciferin

Main publications

1. Kotlobay AA et al (2023). Structure elucidation of Keroplatus (Diptera:Keroplatidae) fungus gnat oxyluciferin. Biochem Biophys Res Comm 676 (8), 1-5.